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教學大綱


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審定:無
翻譯:柯佳惠(簡介並寄信)
編輯:朱學(簡介並寄信)


這學期我們的課程將會涵蓋有關內耳的遺傳與分子生物學方面令人興奮的發展。為了參與課程的非分子生物學背景的學生,我們將於課程一開始先上幾堂涵蓋理論與技術的基礎分子生物學與遺傳學。我們也將會有一堂回顧溫習內耳生理學的課。我們將會研讀到可用來找尋「耳聾基因」的技術,還有近年來所找到對於內耳正常運作甚為重要的基因與蛋白質。我們將涵蓋有關於內耳發育與毛細胞再生的主題。另外的課程將會擴展到近年來此領域的發展,譬如結合蛋白、肌動蛋白、轉錄因子、胞外基質蛋白與離子通道。我們也將會討論可被用來進一步研究內耳功能的技術,譬如胚幹細胞的使用。課程主要負責老師將會講授近一半的課程,而其他部分則會由來自波士頓地區研究內耳/耳聾方面的專家來授課。

這年的課程將包含一個實作課程部分,每位學生將會操作在找尋基因時所要用到的基礎分子生物學技術。學生的研究計畫將於學期末於課堂中發表。此外,每位學生要上台報告研究論文,這些論文必須深入地涵括某些在課堂已討論過的主題。



實驗室

這年的課程將包含一個實作課程部分,每位學生將會操作找尋基因所要用到的基礎分子生物學技術。每個學生將會有他/她自己的小型研究計畫。學生的研究計畫結果將會於學期末於課堂上發表。

學生主導式討論會議

每學期兩次,每位學生將報告曾於課堂中有被深入討論的主題的相關研究論文。這些口頭報告是為了能讓學生對於研究人員用來研究聽覺的分子生物學的技術與策略更加熟稔所設計的。

分數

分數將會以學生在課堂的參與表現、學生的實驗室報告與期刊文章報告,和期末測驗為依據。

建議的教科書

有關基礎分子生物學的部分,我們推薦由Bruce Alberts等人所著、由Garland Science於2002年所發行的《細胞的分子生物學》 (Molecular Biology of the Cell)。



During the semester we will cover the exciting advances in the genetics and molecular biology of the inner ear. For the non-molecular biologists in the class, we will begin with a few lectures on basic molecular biology and genetics, covering both theory and techniques. We will also include an inner ear physiology review. We will study techniques for identifying "deafness genes" as well as genes and proteins recently identified as being critical for proper inner ear function. We will cover topics relating to inner ear development and hair cell regeneration. Additional lectures will explore recent advances in the field, studying such topics as syndromic and nonsyndromic hearing loss and the molecules that are responsible for hearing loss, such as connexins, myosins, transcription factors, extracellular matrix proteins and ion channels. We will also discuss techniques, such as the use of embryonic stem cells, used to further investigations into inner ear function. The lead faculty will deliver approximately half of the lectures, with the remaining lectures given by inner ear/deafness research experts from the Boston area.

The course this year will include a hands-on laboratory component, in which each student will perform basic molecular biology techniques for gene discovery. The students' research projects will be presented in class at the end of the semester. In addition, each student will present for discussion papers that cover in depth some of the topics discussed in class.



Lab

The course this year will include a hands-on laboratory component, in which each student will perform basic molecular biology techniques for gene discovery. Each student will have his/her own mini-research project. Results of students' research projects will be presented in class at the end of the semester.

Student Lead Discussion Sessions

Twice during the semester, each student will present for discussion papers that cover in depth the topics discussed in class. These presentations are designed to familiarize students with techniques and strategies used by investigators to study the molecular biology of the auditory system.

Grades

Grades will be assigned based upon student participation in class, student presentations of lab work and journal articles, and a final exam.

Recommended Text

For coverage of basic molecular biology, we recommend Molecular Biology of the Cell by Bruce Alberts et al., 2002, Garland Science.


 
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